sandwich elisas for vegf  (R&D Systems)

Journal: Scientific Reports

Article Title: The heterogeneity of NOTCH1 to tumor immune infiltration in pan-cancer

doi: 10.1038/s41598-024-79883-1

Figure Lengend Snippet: The correlation of NOTCH1 with angiogenesis and angiopoietins. The KEGG pathways of NOTCH1 and its ligand protein of the GSEA gene set; Different colors respectively represent the enriched signal pathway, and the deeper the color gradient, the smaller the correlation of pvalue value (A) ; VEGF expression in the supernatant culture medium of three kind cells were detected by ELISA; * means p < 0.05 (B) ; The correlation between NOTCH1 and genes of angiogenesis; The correlation between NOTCH1 and genes of angiopoietin, p < 0.05 had significant correlation (C) .

Article Snippet: The VEGF content of cell culture supernatant was measured using human VEGF DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Cells

Article Title: The Effects of the Pneumonia Lung Microenvironment on MSC Function

doi: 10.3390/cells13181581

Figure Lengend Snippet: Cytokine measurement in MSC-CM exposed to pooled lung homogenate. ( a ) Lung homogenate exposure significantly increased IL-8 concentration in MSC-CM; the level of IL-8 was significantly higher in E. coli groups at 100 and 200 µg/mL than in sham groups at the same concentrations. ( b ) The level of IL-6 in MSC-CM was significantly higher in the E. coli groups than in the control and sham groups at all concentrations. ( c ) Lung homogenate exposure significantly increased the release of MCP-1 in MSC-CM; the level of MCP-1 was significantly higher in E. coli groups at 200 µg/mL than in sham groups at the same concentration. ( d ) Lung homogenate exposure significantly increased the level of VEGF-A in MSC-CM; the level of VEGF-A was significantly higher in E. coli groups at 50 and 100 µg/mL than in sham groups at the same concentrations. ( e , f ) The level of TNFR1 and TGF-β1 in MSC-CM: No significant difference was detected between any groups. Representative results of three independent experiments are shown as mean ± SD and analysed by two-way ANOVA with Šídák’s multiple comparisons test. *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001; ns: no significance.

Article Snippet: The MSC conditioned media (MSC-CM) was analysed to quantify different cytokines by ELISA kits, including interleukin 8 (IL-8, cat. no. DY208 R&D Systems™, Abingdon, UK), interleukin 6 (IL-6, cat. no. DY206 R&D Systems™, Abingdon, UK), monocyte chemoattractant protein 1 (MCP-1, cat. no. DY279 R&D Systems™, Abingdon, UK), vascular endothelial growth factor (VEGF-A, cat. no. DY293B R&D Systems™, Abingdon, UK), tumour necrosis factor receptor 1 (TNFR1, cat. no. DY225 R&D Systems™, Abingdon, UK), and transforming growth factor beta-1 (TGF-β1, cat. no. DY240 R&D Systems™, Abingdon, UK).

Techniques: Concentration Assay, Control